LOCUS       ISCR1-armA_unit         4703 bp    DNA     linear       10-NOV-2020
DEFINITION  Citrobacter freundii.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Unclassified.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISCR1-armA_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
     mobile_element  1..4703
                     /gene="ISCR1-armA unit"
                     /product="Putative resistance unit: ISCR1-armA unit"
     mobile_element  complement(1..2154)
                     /gene="ISCR1"
                     /product="Insertion sequence: ISCR1"
     CDS             381..1922
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="tnpA for ISCR1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASL
                     EAQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGAR
                     RMAESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIK
                     KAGYTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYD
                     ELNTLAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGP
                     QQGRKVFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPA
                     VSEKRLALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVF
                     APNSKHRVQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVC
                     EHCGGHVKVIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQ
                     SRLFD"
     repeat_region   2134..2154
                     /gene="oriIS"
                     /product="oriIS of ISCR1"
     mobile_element  2155..3051
                     /gene="truncated ISEc28"
                     /product="Insertion sequence: truncated ISEc28"
     misc_feature    2159..2995
                     /gene="truncated tnpA"
                     /product="Truncated ISEc28 transposase"
     repeat_region   3033..3051
                     /gene="IRR_ISEc28"
                     /product="ISEc28 inverted repeat right"
     CDS             3321..4094
                     /gene="armA"
                     /codon_start=1
                     /transl_table=11
                     /product="Ribosomal RNA methyltransferase"
                     /translation="MDKNDVVKKILESKKYENLDSDIVEKVVSISEKKYKLKEVENYS
                     KKKLHQIWGSYYSAYPNWDKLLKKYNQGQLSIEDLLKIHSSTNERVATLNDFYTYVFG
                     NIKHVSSILDFGCGFNPLALYQWNENEKIIYHAYDIDRAEIAFLSSIIGKLKTTIKYR
                     FLNKESDVYKGTYDVVFLLKMLPVLKQQDVNILDFLQLFHTQNFVISFPIKSLSGKEK
                     GMEENYQLWFESFTKGWIKILDSKVIGNELVYITSGFQK"
     CDS             complement(4075..4356)
                     /gene="mnmA"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /translation="MLYVVQGKDNPKLWKNIVSVSELHLINETSLLNNNYTASIRYRS
                     QDTPVKVTQNENGYIFEFSAPQWAPAVGQSLVLFQENECLGGGVISEIH"
BASE COUNT     1319 a   1102 c   1148 g   1134 t
ORIGIN
        1 aatatctcct tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa
       61 agttttgtgt gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag
      121 ctcgacgact accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat
      181 aacagttggg gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt
      241 ctcgctcaac cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta
      301 gcgatgtcta gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac
      361 agtatcagcg gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca
      421 ctcaaacaaa cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg
      481 agcagcacta cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct
      541 acatccaaca agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc
      601 gggttcgctg cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg
      661 gcttttgccc tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg
      721 aagtcttccc caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct
      781 ttttgctggc tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac
      841 tctcaactca tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag
      901 tgactcttat ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt
      961 ttctcgatgg tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg
     1021 cacccactta cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct
     1081 gcatggaaaa gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag
     1141 agggcgaaga cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg
     1201 tcggccccca gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata
     1261 aagccgactc aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga
     1321 tggccgaagc gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag
     1381 cggtttcaga aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa
     1441 ctccgtaccg caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac
     1501 tcgctgcgtt ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac
     1561 cgaacagcaa acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat
     1621 cggaaggtct cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga
     1681 cctggatgca acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg
     1741 gcggtcacgt caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca
     1801 agcatctgaa acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag
     1861 aacgagcgcc gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact
     1921 gacgacccca aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag
     1981 tttgtatctc tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc
     2041 ggtttaggct ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt
     2101 caggtaagat acttcggatg aggagcaaaa aggtggttta tacttcctat acccctggat
     2161 ggacgaacaa gccattaagc agtggcattg cctggtccac catggccgcc gagggagagg
     2221 tttccactac agtgacaccg ccattgagac ggccctgatg ctaaaaggct tgttcaagtt
     2281 gccgctgcgg gcgctagaag gcttcattaa ctcgttgttc caactgatgg cggtgccttt
     2341 gcagtcgccg gactatagct gtatcagcaa gcgcgccaag accgtcgaca tcaagtaccg
     2401 cctccccagc caaggcccgg tggctcactt ggtcattgat gccaccggcc tcaaggtcta
     2461 tggcgaaggc gaatggaaaa ttcgcaaaca cggcaaggag aagcgacgag tgtggcgcaa
     2521 gctccacttg gcggtggatg ccacaaccca tgcggtcatc gctgccgaag tcagcctgga
     2581 gacggtggcg gataacgagg tgttaccgac gttgctcaac cccttacgac gcaagataaa
     2641 acaggtcagc gccgatggtg cgtatgacac caaggcctgt catgcgctac tgaagaaaaa
     2701 gggggcgaaa gccaccatac cgccgagaaa aaatgcggca ccgtgggagg aaggccatcc
     2761 ccgcaacgag gcggtgacgg cgctcaaggc cggtgaactg aagcaatgga agaaagactc
     2821 cggctatcac cagcgctcga tagccgagac cgccatgtac cggttcaagc aactcatcgg
     2881 gccaacgctg agcctgcgga actacaacgc ccaggtgggc gaaatcctgg ctggcgtgaa
     2941 ggtgatgaac aagctgatag ggcttggtat gcctgttcgc cagccagtga attgagtggt
     3001 atcaacgggt tggggaacgg ccatccagcg acggatttgg tcaacaacgc cgataacacc
     3061 tcgaaatcgg agtttatatg gcttcgtggg ggagttatgt gtttgagctt tgtcgcatta
     3121 acgcgaatca ggggttgacg gcagaaatgg actggaatta cacttcttag agtcggcgtt
     3181 gccggaaaat tctgattgga ttagttgttc ggggtgcgaa aacagtcgta gttcggggga
     3241 aaaaccgaat tttgacccga aaccgcaaaa aacccgaaat gacagttcct atcaaatact
     3301 taacttgtag gttctttcaa atggataaga atgatgttgt taagaagata cttgaatcaa
     3361 aaaagtacga aaaccttgat tcagatattg ttgaaaaggt tgtttccatt tctgagaaga
     3421 aatataaatt aaaggaagtt gagaattatt ctaaaaagaa attgcatcaa atatgggggt
     3481 cttactattc tgcctatcct aattgggata aattattaaa aaagtacaat caggggcagt
     3541 tatcaataga agatttacta aagattcatt cttcgacgaa tgaaagagtc gcaacattaa
     3601 atgactttta cacttatgta tttggaaata tcaaacatgt ctcatctatt ttagattttg
     3661 gttgtggctt caatccatta gctttatacc aatggaatga aaatgaaaaa ataatatatc
     3721 atgcatacga tattgataga gctgagatag cttttttgag tagcattatt gggaagttaa
     3781 agacgacgat aaagtatagg tttttgaata aagagagtga tgtctacaaa ggtacttatg
     3841 atgtagtatt ccttttaaag atgcttcctg tgctaaaaca gcaagatgta aatatcttgg
     3901 atttcctaca gctttttcat actcaaaact ttgtaatatc ttttccaata aagtctttat
     3961 ctggaaagga gaagggaatg gaagagaatt accagctatg gtttgaatct tttacaaaag
     4021 gttggataaa aatccttgat tcgaaggtta tagggaatga gttagtatat attactagtg
     4081 gatttcagaa ataactccgc ctccaaggca ctcattttct tggaaaagta ctaaactctg
     4141 tccaacggct ggagcccatt gtggtgcaga aaattcaaat atatatccat tttcattctg
     4201 ggttactttt actggtgtat cctgacttct ataacggata gatgctgtat agttattatt
     4261 aagtaatgaa gtttcgttta tcaagtgcaa ttctgaaaca cttactatgt ttttccaaag
     4321 ttttggatta tcttttcctt ggacaacata gaggatattt tgaggaaaag ataggtttca
     4381 actacagttt agaaggttaa aaacaaatgc atgcatggct caaaagcaat ctagggaagg
     4441 tgcgaataag cggggaaatt cttctcggct gactcagtca tttcattttt tcctgtttga
     4501 gccgtttttt ctcccgtaaa tgcactgtat accgatatgg gcagggcgaa gcgctaaagt
     4561 acaacaatct tattgttagc tattgtcttg gcgttccaga aaatctttaa actcaatagt
     4621 ataaagccgt aacagcacca tggttaacgc aaaaataatt ggcccataaa tcaaaccaat
     4681 taaaccaaag agttgcaaac cac
//
